Hi
The low pH and its interaction with PIP1 can be conflicting. The low
pH can modify the interaction site.
Nevertheless, one good way to study protein stability is thermal shift assay.
You can read this publication too : PubMed ID 16604423. (Rapid
determination of protein solubility and stability
Dear all,
Sorry for this off-topic question.
I am studying a peripheral membrane protein (13kDa) by liquid state
NMR, which is stable at pH 9.0 (50mM CHES pH9.0, 150mM NaCl and 1mM
DTT). It crashes when I lower pH of buffer. The addition of 50mM Arg +
Glu works to stabilize the protein at low pH,
