Often you can also avoid this skin formation by adding a bit of your reservoir
solution first to make it a larger droplet.
Then the microtools as mentioned earlier or just two loops
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemis
gt; > Message du 25/11/10 15:56
> > De : "Rick"
> > A : CCP4BB@JISCMAIL.AC.UK
> > Copie à :
> > Objet : [ccp4bb] Tough 'shell' on disturbed drop
> >
> > Dear CCP4
> >
&g
> De : "Rick"
> A : CCP4BB@JISCMAIL.AC.UK
> Copie à :
> Objet : [ccp4bb] Tough 'shell' on disturbed drop
>
> Dear CCP4
>
> I looped a v.thin rod emerging from a cluster of v.thin rods that grew in
> 29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic acid,
The shell may be denatured protein. Remove the protein from the
experiment and the problem will likely go away.
On 11/25/10 09:45, Rick wrote:
Dear CCP4
I looped a v.thin rod emerging from a cluster of v.thin rods that grew in
29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic acid, sodium di
Hi,
You're working with a very 'rich' crystallization condition. It probably was
supersaturated or close to super-saturated with respect to something, and
that something crashed out on the surface (where liquid contacted air)
forming a crust. Your loop (while perfectly clean) can also be the sourc
Dear CCP4
I looped a v.thin rod emerging from a cluster of v.thin rods that grew in
29%PEG1500 and 0.1M SPG buffer at pH7.5 (succinic acid, sodium dihydrogen
orthophospate and glycine). The loop i used had been washed more than 10 times
with deionised water (so assumed as 'clean'). The crystals
