Re: [ccp4bb] Thermofluor experiment

Hi, I am interested in using the thermofluor to assess the stability of my protein in different buffers. Can anyone recommend a vendor that supplies buffer screens, possibly in 96 well format? Not crystallization buffers, just ordinary storage buffers. Thanks brenda Quoting Andreas Förster

Re: [ccp4bb] Thermofluor experiment

ECTED] On Behalf Of Andreas Förster Sent: Wednesday, February 13, 2008 4:01 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Thermofluor experiment Dear Kornelius, I found the idea of doing Thermofluor on membrane proteins really intriguing - for identifying the best buffer and detergent, seco

Re: [ccp4bb] Thermofluor experiment

Dear Kornelius, I found the idea of doing Thermofluor on membrane proteins really intriguing - for identifying the best buffer and detergent, secondary detergents, even for checking crystallization drops that stayed clear. (This latter experiment should theoretically be possible with large dr

Re: [ccp4bb] Thermofluor experiment

Kornelius, have a look at the following paper: Yeh AP, McMillan A, Stowell MH.

[ccp4bb] Thermofluor experiment

Dear all, a question very related to the discussion before. I have been reading the papers about the thermofluor experiment with great interest. I wonder what people think about the underlying principles/ideas and the success that the method yielded in their own labs for crystallization or re