Dear Samer,
that is quite an important point for construct design especially in
the context of signal sequence. That is not a trial task, but based on
the information you have shared with us I would assume that your
protein does not get properly processed in the ER if you cannot detect
it by Flow c
Dear All,
Sorry to be disturbing you all with my inquiry. I am tagging a membrane protein
(MHC I) just after the Signal peptide with either HA-tag or Myc-tag and
expressing it in mammalian cells. I am able to detect that protein by
immunoprecipitation followed by Western blotting using anti-Myc
