Hi Ruben,
I have never used TMAO myself but this paper might give you a better idea:
Jeruzalmi, D. & Steitz, T. Use of organic cosmotropic solutes to
crystallize flexible proteins: application to T7 RNA polymerase and its
complex with the inhibitor T7 lysozyme. J Mol Biol, 1997, 274, 748-56
T
Hi all!
Just want to ask whether some of you has experience with TMAO (or
similar conformation stabilizers) for setting up crystallization
screenings?
Which concentration range would you use? Would you add TMAO to the
protein tube and afterwards add the mixture to the plates?
Just wanted to know yo
