Re: [ccp4bb] THANK YOU: Crack-resistant tubes for centrifugation

2012-02-02 Thread Artem Evdokimov
P.P.S. it also matters a LOT how you fill the tubes. Leaving too much air gap on the top is actually very likely to cause crushing of the plastic. On Thu, Feb 2, 2012 at 6:18 PM, Artem Evdokimov wrote: > Clearly, it's better to use shape-matched rotors (I sort of assumed > that you do that alread

Re: [ccp4bb] THANK YOU: Crack-resistant tubes for centrifugation

2012-02-02 Thread Artem Evdokimov
Clearly, it's better to use shape-matched rotors (I sort of assumed that you do that already!); however the BD/Falcon polyethylene tubes (conical ends) will actually change shape (flow) when placed into the round-ended rotors, if the speed is high enough -- and most of the time the tubes survive th

[ccp4bb] THANK YOU: Crack-resistant tubes for centrifugation

2012-02-02 Thread Raji Edayathumangalam
Hello Everyone, Many many thanks to all the folks who responded to my question with very good suggestions. Here's a very quick and dirty summary of the various tubes and rotors that people use without any issues: (1) 50ml Nalgene tubes for an SS-34 rotor (2) Shape-matched new Fiberlite rotors (3)