mber, TEV works even better if the site is ENLYFQ-S.
Zhijie
- Original Message -
From: "Jacob Keller"
To:
Sent: Friday, June 05, 2009 6:29 PM
Subject: Re: [ccp4bb] TEV nucleotude sequence with restriction site
I checked out the Sheffield et al paper, and the restriction
Does anybody have a TEV-protease-site-coding nucleotide sequence with a
commonly-used restriction site in it, preferably right at the end?
Alternatively, does some somebody know of a program to determine all
equivalent codon permutations for a small coding region, filtered for
resulting restric
60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-kell...@northwestern.edu
> ***
>
> - Original Message - From: "Cynthia Kinsland"
> To: "Jacob Keller"
> Cc:
> Sent: Friday, June 05, 2009 5:19 PM
> Subject
sage - From: "Cynthia Kinsland"
To: "Jacob Keller"
Cc:
Sent: Friday, June 05, 2009 5:19 PM
Subject: Re: [ccp4bb] TEV nucleotude sequence with restriction site
I'm not quite sure what you want, but I have a series of vectors
encoding various N-terminal tags and
quot;
To: "Jacob Keller"
Cc:
Sent: Friday, June 05, 2009 5:19 PM
Subject: Re: [ccp4bb] TEV nucleotude sequence with restriction site
I'm not quite sure what you want, but I have a series of vectors encoding
various N-terminal tags and fusions, all followed by a TEV site. They
I'm not quite sure what you want, but I have a series of vectors
encoding various N-terminal tags and fusions, all followed by a TEV
site. They have an MCS standard to many pET vectors. Therefore, they
are designed to clone your gene in using the NdeI site at the 5' end
(which will, after
You seem to be describing the MCS found in many TEV-site-containing
expression plasmids (am I missing something?) E.g., look at the
sequences in Sheffield et al., Protein Expression and Purification
15, 34 –39 (1999) (let me know if you want a PDF, I don't want to
send it to the whole bb)
Dear Crystallographers,
Does anybody have a TEV-protease-site-coding nucleotide sequence with a
commonly-used restriction site in it, preferably right at the end?
Alternatively, does some somebody know of a program to determine all
equivalent codon permutations for a small coding region, filte
