al Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of David
Briggs
Sent: 12 August 2010 20:22
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Suggestions for Reducing Protein Precipitation
Hi Matt,
Have you tried changing the pH? Is it possible that at pH 8 you are
Hi all.
I found that reference. :0)
Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):833-42. Epub 2006 Jun 20.
Assessment of a preliminary solubility screen to improve
crystallization trials: uncoupling crystal condition searches.
Izaac A, Schall CA, Mueser TC.
http://www.ncbi.nlm.nih.gov/
Hi Matt,
Have you tried changing the pH? Is it possible that at pH 8 you are at
the pI of your protein (i.e. where it has zero net charge and is at
its least soluble) ?
I have also read a paper (can't find the reference right now) where
the authors precipitated their protein by dialysing into wat
B@JISCMAIL.AC.UK
Subject: [ccp4bb] Suggestions for Reducing Protein Precipitation
Hi.
I am working with a protein that has difficulties staying in solution when
concentrated. The buffers that I have been using contain 20 mM Tris pH 8.0,
5 mM BME (or 2 mM DTT), 10% Glycerol, and NaCl from 50 mM
cp4bb] Suggestions for Reducing Protein Precipitation
Hi.
I am working with a protein that has difficulties staying in solution when
concentrated. The buffers that I have been using contain 20 mM Tris pH 8.0, 5
mM BME (or 2 mM DTT), 10% Glycerol, and NaCl from 50 mM up to 2 M. The protein
seems
Hi.
I am working with a protein that has difficulties staying in solution when
concentrated. The buffers that I have been using contain 20 mM Tris pH 8.0,
5 mM BME (or 2 mM DTT), 10% Glycerol, and NaCl from 50 mM up to 2 M. The
protein seems fine to dialyze into low salt buffer (50 mM NaCl) when
