Evdokimov
Date: Saturday, February 19, 2022 at 2:28 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Strategies for increasing Trp content of proteins
Yes we chose the sites based on predicted or known structure, to be sure. No
that was not a published thing, curse of commercial work :(
Happy to
in board on behalf of Casper
> Wilkens <5d34ab0aef35-dmarc-requ...@jiscmail.ac.uk>
> *Date: *Saturday, February 19, 2022 at 2:19 AM
> *To: *CCP4BB@JISCMAIL.AC.UK
> *Subject: *[ccp4bb] Sv: [ccp4bb] Strategies for increasing Trp content of
> proteins
>
> *WARNING:* This messag
19, 2022 at 2:19 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: [ccp4bb] Sv: [ccp4bb] Strategies for increasing Trp content of proteins
WARNING: This message has originated from an External Source. This may be a
phishing expedition tha
on behalf of Casper Wilkens
<5d34ab0aef35-dmarc-requ...@jiscmail.ac.uk>
Date: Saturday, February 19, 2022 at 2:19 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Sv: [ccp4bb] Strategies for increasing Trp content of proteins
WARNING: This message has originated from an External Source. Th
okimov
*Sendt:* 19. februar 2022 02:12:50
*Til:* CCP4BB@JISCMAIL.AC.UK
*Emne:* Re: [ccp4bb] Strategies for increasing Trp content of proteins
We have had success placing single trp on the outside of proteins, as
weird as it sounds it works pretty well. If your protein is easy to
express you can
r_Wilkens
<https://www.cazypedia.org/index.php/User:Casper_Wilkens>
Fra: CCP4 bulletin board på vegne af Artem Evdokimov
Sendt: 19. februar 2022 02:12:50
Til: CCP4BB@JISCMAIL.AC.UK
Emne: Re: [ccp4bb] Strategies for increasing Trp content of proteins
We ha
We have had success placing single trp on the outside of proteins, as weird
as it sounds it works pretty well. If your protein is easy to express you
can try a few choices.
If your protein is difficult to express, a fusion with a trp-positive
domain or with gfp may be a better option.
There are m
Dear CCP4BB,
We are working on a protein that has no Trp residues, which makes
chromatography challenging due to the low absorbance at 280 nm (Abs 0.1% =
0.104). Does anyone have experience using mutagenesis to increase the Trp
content of proteins?
Thanks,
Jack Tanner
--
John J. Tanner
Profe
