Re: [ccp4bb] Stabilization of crystals and ligand exchange

2012-10-18 Thread Patrick Shaw Stewart
I say (of course I would!) why not try co-crystallization with random microseeding using the crystals with the original ligand? It usually allows you to control the number of crystals per drop too On 18 October 2012 15:59, Dmitry Rodionov wrote: > Hi Sabine, > > Glutaraldehyde crosslinking wor

Re: [ccp4bb] Stabilization of crystals and ligand exchange

2012-10-18 Thread Julia Griese
Hi Sabine, I also have good experience with crosslinking crystals with glutaraldehyde to stabilise them for soaks with ligands that were only soluble in DMSO (http://dx.doi.org/10.1016/j.jmb.2006.06.007). More often than not it destroys the crystals, I think, but it's worth a try. If you can

Re: [ccp4bb] Stabilization of crystals and ligand exchange

2012-10-18 Thread Dmitry Rodionov
Hi Sabine, Glutaraldehyde crosslinking worked pretty good for various soaks in my experience. J. Appl. Cryst. (1999). 32, 106-112[ doi:10.1107/S002188989801053X ] A gentle vapor-diffusion technique for cross-linking of protein crystals for cryocrystallography C. J. Lusty Best regards,

Re: [ccp4bb] Stabilization of crystals and ligand exchange

2012-10-18 Thread Sabine Schneider
herman.schreu...@sanofi.com [herman.schreu...@sanofi.com] Sent: Thursday, October 18, 2012 10:32 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Stabilization of crystals and ligand exchange Hi Sabine, The easy experiment to start with, is to take your best conditions (nice looking crystals, no

Re: [ccp4bb] Stabilization of crystals and ligand exchange

2012-10-18 Thread Boaz Shaanan
: [ccp4bb] Stabilization of crystals and ligand exchange Hi Sabine, The easy experiment to start with, is to take your best conditions (nice looking crystals, no diffraction) and instead of overnight wait 4-8 weeks. Sometimes ligand exchange is slow, or the ligand induces a conformational change which

Re: [ccp4bb] Stabilization of crystals and ligand exchange

2012-10-18 Thread Herman . Schreuder
- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Sabine Schneider Sent: Wednesday, October 17, 2012 6:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Stabilization of crystals and ligand exchange Hi everyone, I am trying to get the structure of a protein-ligand complex

[ccp4bb] Stabilization of crystals and ligand exchange

2012-10-17 Thread Sabine Schneider
Hi everyone, I am trying to get the structure of a protein-ligand complex were I need to exchange the ligand which it co-crystallises nicely with. Problem: either they crack, disolve, turn brown,... OR they still look very nice, well shaped but do not show a single reflection at the synchrotr