Dear Maria -
Lipid diffraction resembles powder diffraction rather than discrete spots -
you can see examples in Cherezov's 2002 Biophys J article, for example - so
you wouldn't mistake them for protein diffraction. If not a case of
overlooked predictions as Kay suggests, you have probably crystall
Dear Maria,
maybe a case of underpredicted/overlooked reflections? It could then be P2x2x2x
instead of I222/I212121. Have you checked the predictions?
best,
Kay
On Fri, 22 May 2020 10:08:40 +, Demou, Maria
wrote:
>Dear all,
>I have a question that may have a straight forward answer, and
This reminds me of something that we crystallized a few years back. It indexed
as I222 (or I2(1)2(1)2(1)) and the cell was quite small; too small for the
protein of interest. Almost certainly a contaminant, but it didn’t show up in
Contaminer. Diffracted like gangbusters, but we never figured ou
Yees - changing from I222 to I21 21 21 wont increase the unit cell volume..
Eleanor
On Fri, 22 May 2020 at 15:47, <0c2488af9525-dmarc-requ...@jiscmail.ac.uk>
wrote:
> I don't know about LCP but it sounds like a case for Contaminer or Simbad.
>
> Jon Cooper
>
> On 22 May 2020 11:08, "Demou, Ma
I don't know about LCP but it sounds like a case for Contaminer or Simbad.Jon CooperOn 22 May 2020 11:08, "Demou, Maria" wrote:
Dear all,
I have a question that may have a straight forward answer, and was wondering if this is a common issue. We have a protein crystallised in I222 space group
Dear Maria,
are you sure, it's I222? From the extinction rules, you can't
distinguish between I222 and I2(1)2(1)2(1).
Best regards,
Dirk.
On 22.05.20 12:08, Demou, Maria wrote:
Dear all,
I have a question that may have a straight forward answer, and was
wondering if this is a common issue.
Dear all,
I have a question that may have a straight forward answer, and was wondering if
this is a common issue. We have a protein crystallised in I222 space group.
This is CRP, but the monomer/pentamer is not predicted to fit in this space
group. Is there a possibility of the lipid cubic phase
