, January 29, 2009 5:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Small lines in diffraction pattern (more info)
Ian and Herman,
does one want to convolute the electron density at all? I was under the
impression that current thinking favors convolution of the model instead, i.e.
placing
So it would seem that the five-fold periodicity in the spot intensities
along the long axis is probably due to a short-range, 34 Ang pseudo-repeat
(the decamer) along the true 170 Ang cell axis (170 = 34 x 5).
I do not think that the streaks are helical layer lines, because the spacing
on the
FILETIME=[831110D0:01C9822E]
Yes I agree with that!
-- Ian
> -Original Message-
> From: Andreas Förster [mailto:docandr...@gmail.com]
> Sent: 29 January 2009 16:26
> To: herman.schreu...@sanofi-aventis.com; Ian Tickle
> Cc: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [cc
Ian and Herman,
does one want to convolute the electron density at all? I was under the
impression that current thinking favors convolution of the model
instead, i.e. placing both the helices in both orientations at partial
occupancy and letting the refinement program figure things out?
An
riet.ova...@chem.kuleuven.be; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] Small lines in diffraction pattern (more info)
Dear Margriet,
From your description and what James Holton wrote, it seems
that you have 2 types of unit cells:
A: with the "sense" strand in position 1 and the "
...@astex-therapeutics.com]
Sent: Thursday, January 29, 2009 12:53 PM
To: Schreuder, Herman R&D/DE; margriet.ova...@chem.kuleuven.be;
CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] Small lines in diffraction pattern (more info)
Hi Herman
Aren't detwinning methods appropriate only in th
leuven.be; CCP4BB@JISCMAIL.AC.UK
> Subject: RE: [ccp4bb] Small lines in diffraction pattern (more info)
>
> Dear Margriet,
>
> From your description and what James Holton wrote, it seems
> that you have 2 types of unit cells:
> A: with the "sense" strand in position 1
argriet Ovaere
Sent: Thursday, January 29, 2009 10:45 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Small lines in diffraction pattern (more info)
Dear all,
There were some comments about detector issues, but these can be ruled
o
The 34-34-34 cell does not predict all the spots, does it?
from the diffraction pattern it seems only the 34-34-170 or 34-34-340
cell can predict all spots, so the structure should be solved in the
one that predicts all spots.
The procedure I would use is to take a 180º dataset, sacrificing so
Dear all,
There were some comments about detector issues, but these can be
ruled out, to my opinion, since the lines appeared on different
beamlines.
Default settings of mosflm (spot picking) finds the cell 34 34 34 90
90 90 (pointless indicating P41212)
Structure was solved by SAD ph
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