Re: [ccp4bb] Skimming crystal

2024-05-30 Thread Patrick Shaw Stewart
Hi 白雪慧 There's another solution, which I have never known to fail. Set up your conditions with everything in the drop except for the butanol in one of our "Vapor Batch" plates. Cover the drops with oil - we use light mineral oil - although I'm sure heavy would be just as good. Then put the same

Re: [ccp4bb] Skimming crystal

2024-05-30 Thread Artem Evdokimov
Hello This is a common issue with volatile organics in the drop. Solutions that have worked in the past include: 1. covering the drop in oil (some people prefer light oil, I personally prefer heavy/viscous oil, as it makes harvesting easier) 2. diluting the drop with a much larger volume of mothe

Re: [ccp4bb] Skimming crystal

2024-05-30 Thread Mark J. van Raaij
We had exactly the same thing happening with some crystals grown in tBuOH. The evaporation of tBuOH leads to currents in the drop and crystals tumbling about. Our first solution was patience and persistence, trying to trap a goodlooking crystal near the edge and loop it out. After a time however,