Can anyone suggest me a protocol for silver-staining the PAGE that is
already stained with Coomassie.
There is absolutely nothing wrong with silver staining of a
Coomassie-stained gel without destaining. It only prevents "blowout" of
most intense bands already well-visible with Coomassie. The
trick I hadn't heard of before!
>
>
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Artem Evdokimov
> Sent: Friday, September 16, 2011 10:27 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Silver stain
Now that's a nifty trick I hadn't heard of before!
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Artem Evdokimov
Sent: Friday, September 16, 2011 10:27 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Silver staining Coomass
Destain it really well. One easy peasy option is to put the gel in water and
add 1 or 2 ml of cheap Q sepharose to it then boil. Gel will destain in 2-3
minutes of boiling.
On Sep 16, 2011 5:10 AM, "K Singh" wrote:
> Dear All,
> Can anyone suggest me a protocol for silver-staining the PAGE that is
On Fri, 2011-09-16 at 15:28 +0530, K Singh wrote:
> Dear All,
> Can anyone suggest me a protocol for silver-staining the PAGE that is
> already stained with Coomassie.
> Kris
Just destain it and then use the standard silver-staining protocol. If
for some reason you want to have both stainings sup
Dear All,
Can anyone suggest me a protocol for silver-staining the PAGE that is
already stained with Coomassie.
Kris
