Hi Kerstin,
"Should I refine a low-resolution dataset 6 A": usually we refine a model
of the crystal structure against measured data set.
Besides, technically you can do this, and I hope other replies will help
you set correct expectations about the realities of the process.
At this resolution y
. Juni 2015 13:19
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Should I refine a low-resolution dataset 6 A
Hi Experts,
Last year I collected a dataset at 6 A of 2600 aa multi-domain protein.
Resolution range 50 - 6 (6.7-6)
Wilson B 370 A^2
Reflection 34120 (12240)
Rmeas 0.2 (4.4)
Rpim 0.06(1.2)
I
This is a very tough resolution to be working at, but if you want to take it on
I'd suggest that xMDFF
(http://www.ks.uiuc.edu/Training/Tutorials/science/mdff/tutorial_mdff-html/node8.html)
starting from a good homology model is more likely to yield results. Shameless
self-promotion: I've been
Hi Experts,
Last year I collected a dataset at 6 A of 2600 aa multi-domain protein.
Resolution range 50 - 6 (6.7-6)
Wilson B 370 A^2
Reflection 34120 (12240)
Rmeas 0.2 (4.4)
Rpim 0.06(1.2)
I-sigI 9 (1)
CC1/2 0.9 (0.5)
I have the following questions in my mind.
1. Does it make any sense
