Re: [ccp4bb] Sea Urchin like crystals

2014-05-21 Thread Almudena Ponce Salvatierra
Hi Venkat, I think there are maybe a few things you could do. - Have you tried to lower the concentration of your protein? Maybe these clusters appear like so because there is little time for better ordered growth. If you decrease the protein concentration, supersaturation will occur slower and y

Re: [ccp4bb] Sea Urchin like crystals

2014-05-21 Thread Lorenzo Finci
May 2014 15:28:16 +0530 From: venkatda...@gmail.com Subject: [ccp4bb] Sea Urchin like crystals To: CCP4BB@JISCMAIL.AC.UK Dear All, I'm working on 24.3 KDa protein (pI 4.84) with (Asp + Glu): 36 and (Arg + Lys): 16. Protein is in buffer Tris (10mM), pH 7.5. I got sea urchin like crystal clu

Re: [ccp4bb] Sea Urchin like crystals

2014-05-21 Thread MARTYN SYMMONS
ok that up;) ) .  My feeling is fibrous protein is not a good crystallization lead.   But maybe you have had better luck    Martyn     From: venkatareddy dadireddy To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, 21 May 2014, 10:58 Subject: [ccp4bb] Sea Urchin lik