Hi Venkat,
I think there are maybe a few things you could do.
- Have you tried to lower the concentration of your protein? Maybe these
clusters appear like so because there is little time for better ordered
growth. If you decrease the protein concentration, supersaturation will
occur slower and y
May 2014 15:28:16 +0530
From: venkatda...@gmail.com
Subject: [ccp4bb] Sea Urchin like crystals
To: CCP4BB@JISCMAIL.AC.UK
Dear All,
I'm working on 24.3 KDa protein (pI 4.84) with (Asp + Glu): 36 and (Arg + Lys):
16. Protein is in buffer Tris (10mM), pH 7.5. I got sea urchin like crystal
clu
ok that up;) ) . My feeling is
fibrous protein is not a good crystallization lead.
But maybe you have had better luck
Martyn
From: venkatareddy dadireddy
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, 21 May 2014, 10:58
Subject: [ccp4bb] Sea Urchin lik