Hi Meg,
I think with your pi you may also test a Q-Sepharose at neutral to slightly
alkaline pH. Maybe your protein is more comfortable with this condition
compared to a pH of 4.5. As mentioned befor your protein is most likely
precipitated on the column.
Best regards
Christian
Am Dienstag
Hi Meg,
If the bigger peak is appearing when you wash with 1M NaOH, i think your
protein is precipitated on the the column. If you have your protein
relatively pure after the precedent step, maybe you can try different
buffer. You can probably find a better buffer than this one. If your
prot
Dear All,
We used SP sepharose high performance as second stage Ion exchange
chromatography for polishing the product. We did get pure product but yield
obtained was mere 25%. Our protein has a pI of 5.5 - 6.0 and we had used 25
mM Na Acetate buffer pH 4.5 for loading and same buffer with 1M NaCl
