You can include urea in your gel to resolve ~5 kDa without using
gradient gel. I am sorry that I forgot what was the percentage. Please
take a look at "Gel Electrophoresis of Proteins: A Practical
Approach". Or you can try the premade gradient gel from Invitrogen.
http://www.invitrogen.com/etc/medi
Subject: [ccp4bb] SDS-PAGE of peptides
Dear Crystallographers,
does anybody have any good tricks for getting nice, sharp SDS-PAGE bands of
peptides < 10kD?
Regards,
Jacob Keller
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Train
Try high percentage Tricine gels...
Robert Keenan
Assistant Professor
Dept. of Biochemistry & Molecular Biology
GCIS W238
University of Chicago
929 East 57th Street
Chicago, IL 60637
(o) 773.834.2292
(f) 773.834.5416
(e) bkee...@uchicago.edu
http://keenanla
Dear Crystallographers,
does anybody have any good tricks for getting nice, sharp SDS-PAGE bands of
peptides < 10kD?
Regards,
Jacob Keller
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1
