Hi Rongjin,
During the SAXS experiment, have you noticed X-ray induced damage on your
samples that could explain this?
If you are characterizing your protein in solution you may also be able to
pinpoint conformational flexibility using other techniques.
- analytical sedimentation. since you have
n.edu> kgu...@mail.med.upenn.edu
215-573-7260 / 267-259-0082
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Rongjin Guan
Sent: Thursday, July 15, 2010 11:24 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] SAXS on a coiled coil protein
Sorry for a non-ccp4 question.
We have
Sorry for a non-ccp4 question.
We have determined a structure which is mainly a coiled coil motif. The
two helices are from the same protein chain linked by a short turn.
However, the SAXS data indicates that "this protein is probably natively
unfolded or may have very flexible domains and lin
