Hello,
This tag is very hard to release, as it forms an integral part of the
RNAse A. It maybe released with 2M NaSCN but this often results in
denaturation of the carrier protein. This is why the manufacturer strongly
recommends having cleavage site(s) engineered in between the tag and the
carrie
Hi,
I have a membrane protein with a C-terminal S-tag, and there is no cleavage
site for the S-tag (novagen, e.g, thrombin, EK). I wonder whether I still
can use this for purification without cleavage of the S-tag. Here are my
questions:
1) How to release the protein bound to S-tag resin? I noti
