http://www.rostlab.org/services/CHOP/
This is the closest I could find as a server for construct design. The
sample output does not show alignments though, only residue numbers.
It is better to design longer construct than short construct, because
missing critical terminal residues could lead
You would need to incorporate as much information you can find as possible to
make the 'best' prediction of domain boundaries. Typically, I would put
together all the following information in one multiple sequence alignment to
design domain constructs for structural studies.
- Known structural
n these kinds of
predictions. I would be glad to take a look at your sequence confidentially.
Cheers,
Artem
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Jayashankar
Sent: Tuesday, May 20, 2008 1:35 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Right terminal r
You can try:
http://toolkit.tuebingen.mpg.de/hhpred
this gives you a nice domain border prediction based on analog. structures.
Best wishes
Kornelius
On Tue, 20 May 2008 19:34:39 +0200
Jayashankar <[EMAIL PROTECTED]> wrote:
> Dear friends and scientists,
>
> (A pre-Structural biological ques
Dear friends and scientists,
(A pre-Structural biological question.)
I Have a multidomain protein , I know the domain boundaries,
But am still not that rational to what residues a construct should start or
end?
But I have learned from people that changing one residue changes the fate of
the cons
