Dear Vineet
Following points shall be kept in mind.
1.If you have been able to model most of the protein
into the electron density, then its a bit strange.
your refinement parameters suggest that refinement is
still away from convergence.
2.You might be trapped in local minima, and in this
case
Hi all
i m solving a structure at 2.2 A using MIR. rt now my Rfree is 30.55% and
Rcryst is 28.51%. i have already carried out B factor refinement n water has
also been picked up to 2.3 sigma cut off. now i m not able to refine the
structure any further.
The protein has been purified directly from
