] Rfactors stuck very high
Dear James
It seems that at this stage you may not be able to distinguish between these
space groups. As I see they are subgroup of each other (more precisely P1 < C2
< I222 with the cell parameters you have) and it would not be easy to find out
the exact space group a
Dear James
It seems that at this stage you may not be able to distinguish between these
space groups. As I see they are subgroup of each other (more precisely P1 < C2
< I222 with the cell parameters you have) and it would not be easy to find out
the exact space group at this stage. Highest spac
Dear Nat,
thanks for the response.
1) We are very sure the protein crystallized is our protein (very pure reagent
and also verified by washing then dissolving xstals and running SDS-PAGE).
2) I have tried some omit maps and depending which regions are removed, they
generally come back in the
On Sun, Jul 8, 2012 at 2:11 PM, James Garnett wrote:
> I have found a molecular replacement solution in I212121 using an NMR
> structure of the same protein and MR-ROSETTA/PHENIX (PHASER LLG=128
> TFZ=12.3), although I can not refine this below R ~45% and Rfree ~50%. The
> maps look OK in parts
Dear all,
I have some troublesome data to ~2.3A and I hope someone can help. My data
indexes and scales equally well in I222, C2 and P1
I222 a=46.3 b=76.3 c=81.1
C2 a=111.3 b=46.3 c=76.3 beta=133.2 (reset in I2 a=76.3 b=46.3 c=81.1
beta=90.1)
C2 a=94.5 b=76.3 c=46.3 beta=119.6 (reset i
