Re: [ccp4bb] Removing tartrate ions from the active site

2018-04-22 Thread Janet Newman
displacing the tartrate in the active site with your ligand. Janet From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Nemanja Vuksanovic Sent: Sunday, 22 April 2018 12:39 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Removing tartrate ions from the active site Dear All, I&#x

[ccp4bb] Removing tartrate ions from the active site

2018-04-21 Thread Nemanja Vuksanovic
Dear All, I've recently crystallized a protein under a condition containing 1.1 M ammonium tartrate. (I was able to get a few other hits but only these crystals diffract below 2.5 A). Upon refinement, it appears that the electron density in the active site shows a mixture of tartrate and the nativ