To add to your survey:
Yes I have tried it. Yes it does work "better" for glassifying cryobuffers.
I saw Rob Thorne give a talk on this some time ago and immediately went
home to give it a try. I used a cryobuffer (25% (v/v) glycerol; 0.6N
NaOH; 25 mM SeMet) I had been having hit-or-miss ice
It seems to me that this ingenious setup should minimize the
thickness of the cold gas layer, but NOT eliminate it. Effectively,
it should keep the vessel filled up to (or close to) the brim; but
Warkentin et al. show that even when the vessel is filled to the
brim, a significant cold gas
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-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of James
Stroud
Sent: Thursday, February 01, 2007 5:06 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Removal of cold gas layer for
2007 11:06
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Removal of cold gas layer for cryocooling
I propose a simple contraption to minimize the vapor layer in
cryocrystallography, using freezing in the liquid phase. This idea is
based on the findings in the citation below. It is mod
I propose a simple contraption to minimize the vapor layer in
cryocrystallography, using freezing in the liquid phase. This idea is
based on the findings in the citation below. It is modified from
designs used in the pet feeding industry. The novel aspect is that
liquid nitrogen is used rat
Dear everyone,
has anyone tried removing the cold gas layer above liquid nitrogen (or
propane) by blowing or sucking to improve cooling rates in the
cryocooling of crystals. I would like to try and I would be happy if
someone could share some experience.
Florian.
P.S.: there is an interesti
