2012 9:55 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refinement with pseudo-translation
You don't say how many molecules you are looking for?
I would try to first work in SG C2 pretending that the pseudo translation
is 100% of the origin.
Reindex the data as k,l,h, change the SG
-1710
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Eleanor Dodson
[eleanor.dod...@york.ac.uk]
Sent: Sunday, March 25, 2012 9:55 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refinement with pseudo-translation
You don't say how many molecules you are looking for?
I would tr
You don't say how many molecules you are looking for?
I would try to first work in SG C2 pretending that the pseudo translation
is 100% of the origin.
Reindex the data as k,l,h, change the SG to C2 and then redo the scaling
and merging. You will lose all the reflections k+l =2n+1 in your inde
Subject: [ccp4bb] Refinement with pseudo-translation
Dear CCP4bb members
I have a 3.0 Å dataset which has an off-origin peak of height 36% in patterson
map. The peak is at fractional co-ordinates 0, 0.5, 0.5. Data has been indexed
in P2(1)22(1) SG using HKL2000. I have located all the molecules
Dear Shiva
I would be careful with omit maps in the presence of pseudotranslation. You
have to omit bits of molecule from PST related molecules simultaneously,
otherwise you will have bias. If you look at the equation it becomes clear why.
R factors 50/53 is close to random (in the presence of
Dear Shiva,
Refining against weak and strong reflections separately only works if your
intensity distribution is bimodal, because the problem arises from the
assumptions of the scaling protocol (that typically assumes a unimodal
distribution) that leads to underestimating the sigmas of the
Dear CCP4bb members
I have a 3.0 Å dataset which has an off-origin peak of height 36% in patterson
map. The peak is at fractional co-ordinates 0, 0.5, 0.5. Data has been indexed
in P2(1)22(1) SG using HKL2000. I have located all the molecules in asu (as
far as I know) using Molrep with the 'l
