Hi, All,
Thanks for all your replies. I checked the server as Pavel said, and
found that the 2.3A data has strong
anisotropy and other two has severe anisotropy. The recommend resolution
cutoff in the c axis is only
3.4A. I need to read more about the manual but I think this is the problem.
Ho
On 21 May 2008, at 7:00, yang li wrote:
Hi,
I have a structure with 3 different resolutions, 2.3A, 2.4A,
2.5A, the qualities seem normal, not good but also not too bad.
The B factors along a,b,c axis have notable difference, for example
B(a)=80, B(b)=30, B(c)=20. We used molecular
replac
On Tuesday 20 May 2008 22:24, Pavel Afonine wrote:
I agree with Pavel - it is suspicious that TLS refinement
would increase Rfree. That really shouldn't happen.
Are your 3 data sets are truly isomorphous, or could it be that the
one with the bad R factors is really in a lower symmetry space grou
Hi Yang Li,
- the data-to-parameters ratio is not good for individual anisotropic
ADP refinement at this resolution;
- I'm wondering why TLS refinement increases the Rfree... How did you
select the TLS groups? Did you try to use TLSMD for this (thanks Ethan,
it produces TLS groups selections r
Hi,
I have a structure with 3 different resolutions, 2.3A, 2.4A, 2.5A, the
qualities seem normal, not good but also not too bad.
The B factors along a,b,c axis have notable difference, for example B(a)=80,
B(b)=30, B(c)=20. We used molecular
replacement to solve the structure. For the 2.3A data
