Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

: [ccp4bb] Radiation damage to the FAD in enzyme structure Dear colleagues, I am investigating a structure of a FAD-dependent enzyme. The electron density map suggests radiation damage to the FAD. It apparently is different from simple change of the redox state and "butterfly"-like str

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

, 7November, 2017 at 05:16 To: "CCP4BB@JISCMAIL.AC.UK" Subject: Re: [ccp4bb] Radiation damage to the FAD in enzyme structure Dear Ilme, thank you for your answer. We are sure that we have full occupancy. However, we do not know the dose exactly. Nevertheless, after reading the other a

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

, Martin Maly Od: Ilme Schlichting Odesláno: 6. listopadu 2017 12:38:17 Komu: Martin Malý Předmět: Re: [ccp4bb] Radiation damage to the FAD in enzyme structure Hello, Are you sure you had full occupancy to begin with? Can be checked very easily by absorption

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

Dear Martin, A more bleeding-edge type of experiment in the vein David is indicating would be to use a "rotation SFX" protocol to record complete data off one or very few crystals that will be completely exempt from radiation damage, as described in doi:10.1038/nmeth.2962 and in doi: 10.1073/

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

Some alternative interpretations have been suggested, but if you think you are seeing radiation damage, you could try collecting data on several crystals and binning it by dose received. For comparison see: The catalytic pathway of horseradish peroxidase at high resolution. Berglund, et al. (2

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

AC.UK" Subject: [ccp4bb] Radiation damage to the FAD in enzyme structure Dear colleagues, I am investigating a structure of a FAD-dependent enzyme. The electron density map suggests radiation damage to the FAD. It apparently is different from simple change of the redox state and "

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

Dear Martin, we experienced the butterfly-like structure of FAD in monoamine oxidases but this was observed also in other flavoenzymes. Whether this is an intrinsic feature of these enzymes or something due to radation damage is not clear for each case. However, in our experience this bent conform

Re: [ccp4bb] Radiation damage to the FAD in enzyme structure

Dear Martin, a couple of years ago we had a similar problem with FAD degradation. In our case it was not caused by radiation damage, but we saw different ratios of intact and degraded FAD depending on the age of the crystals [Winkler et al., Nat. Chem. Biol. 4, 739-741 (2008)]. Best regards,

[ccp4bb] Radiation damage to the FAD in enzyme structure

Dear colleagues, I am investigating a structure of a FAD-dependent enzyme. The electron density map suggests radiation damage to the FAD. It apparently is different from simple change of the redox state and "butterfly"-like structure. We did not find in literature possible products of radiation