Hi Kornelius,
A possible solution at this resolution would be to use Arcimboldo which
localizes model fragments such as small helices with Phaser and then does
density modification with SHELXE. See:
Nature Methods 6:651-3. "Crystallographic ab initio protein structure
solution below atomic resolu
Hello Kornelius,
In 2 or 3 cases I successfully used a homology model for molecular
replacement using a model generated from the PHYRE server
http://www.sbg.bio.ic.ac.uk/~phyre/.
I know it is not the same as ROSETTA but if there is some sequence homology
you might be lucky...
Best regards
Eike
Hello,
I know at least the following papers on this topic:
---
"High resolution protein structure prediction and the crystallographic
phase problem"
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504711/
"Prospects for de novo phasing with de novo protein models"
http://www.ncbi.nlm.nih.gov/pmc/
Dear all,
I was wondering if anybody has used the ROSETTA software to generate a MR model
that could subsequently being used successfully for a MR solution case.
The sequence of the protein we work with is relatively small, ~ 85 residues.
Crystallization is not very reproducible. Resolution is
