Re: [ccp4bb] RDRP crystal

2019-03-21 Thread #SINGAL BHARTI#
32 PM To: CCP4BB@JISCMAIL.AC.UK; zhangyi19950109 Subject: Re: [ccp4bb] RDRP crystal Hi Zhangyi, You can try using capillary counter diffusion method. Not sure if it can help, but you can give it a try. You can check the following paper. https://www-sciencedirect-com.ezlibproxy1.ntu.edu.sg/sci

Re: [ccp4bb] RDRP crystal

2019-03-21 Thread #SINGAL BHARTI#
to:mala...@ntu.edu.sg> | Web: www.ntu.edu.sg<http://www.ntu.edu.sg/> From: CCP4 bulletin board on behalf of zhangyi19950109 Sent: Friday, March 22, 2019 2:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] RDRP crystal Dear all, A single positive st

[ccp4bb] RDRP crystal

2019-03-21 Thread zhangyi19950109
Dear all, A single positive stranded RNA virus RDRP monomer grows crystals, precipitants (PEG8000) with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution, precipitant concentration increased, many crystal n