L PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, 3 Apr 2007 3:11 AM
Subject: [ccp4bb] Question about cryoprotectant
Hi,
l have a crystal grow at condition screen l 40: 0.1M tri-sodium citrate
dihydrate pH5.6 isoproponal 20%PEG4k 20% and the crystal need a
cryoprotectant, we have used th
Dear Yang Li,
Cryoprotection of crystals is not an exact predictive science - you have
to try a number of things, and in the worst case scenario, none of the
stuff you try may work out.
Assuming that your crystals are OK to start with (capillary mounted room
temp. pattern would confirm that), you
Hi,
l have a crystal grow at condition screen l 40:
0.1M tri-sodium citrate dihydrate pH5.6 isoproponal 20%PEG4k 20%
and the crystal need a cryoprotectant, we have used the 30% glycerol but it
is not good, the mosaicity of
the diffraction pattern is a little high, so anyone knows which is the be
