Here is a snapshot of the anomalous map:
http://imageshack.us/a/img560/1905/anomalousmap.png
The map is contoured to 2.5sigma. They are the strongest peaks in the map.
Not all 7 positions are equally strong.
J.
**
Address:
Joern Krausze
Molecular Stru
@Partha
I have 4 polypeptide chains in the au. The density in the pictures is
located between chain A and C'(symmetry mate of chain C), so it is not
located on a special position
I will prepare and attach a figure of the anomalous map a.s.a.p. In my
memory there was density in all 7 position
Hello Joern,
do you have an image of the anomalous difference map as well? Do you see
the same 7 peaks there, or only the central blob?
Filip
On Thu, Nov 1, 2012 at 11:45 AM, Joern Krausze wrote:
> Dear all,
>
> I have two isomorphous crystals of the same protein. One crystal, let's
> call it
@JISCMAIL.AC.UK] on behalf of Joern Krausze
[jk...@helmholtz-hzi.de]
Sent: 01 November 2012 18:45
To: ccp4bb
Subject: [ccp4bb] Puzzling electron density upon heavy metal soaking
Dear all,
I have two isomorphous crystals of the same protein. One crystal, let's
call it 'derivative', was
Thank you for your efforts!
@BR: Yes, I do have anomalous data which indicate that the density stems
from Co(II) or anything else that shows significant anomalous dispersion
at 1.6083 A. I just cannot picture 7 Co(II) ions forming a perfectly
planar hexagonal structure with one Co(II) in the c
Dear all,
I have two isomorphous crystals of the same protein. One crystal, let's
call it 'derivative', was soaked with 10 mM CoCl2 whereas the other, let's
call it 'original', was not. Both crystals were otherwise grown under
identical conditions (see below) and treated the same. In the derivati
