Re: [ccp4bb] Puzzle with MALS

2011-04-21 Thread zhen zhang
SA standard, to insure system is OK? -Amir From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of zhen zhang [zz2...@columbia.edu] Sent: Thursday, April 21, 2011 5:32 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Puzzle with MALS Dear All, I am havin

Re: [ccp4bb] Puzzle with MALS

2011-04-21 Thread Amir Khan
. Have you run a BSA standard, to insure system is OK? -Amir From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of zhen zhang [zz2...@columbia.edu] Sent: Thursday, April 21, 2011 5:32 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Puzzle with MALS De

[ccp4bb] Puzzle with MALS

2011-04-21 Thread zhen zhang
Dear All, I am having a consistency issue processing my SEC-MALS data. The sample is a 80KD protein which may form a dimer. The experiment I run a year ago shows a major peak at 11.4 ml (GE S200 column), which is calcuated to have a MW of 144kD. There is a minor peak at 10.0 ml. In an effort to