21 21.
Good luck,
Zach
Stern Lab
University of Massachusetts Medical School
From: CCP4 bulletin board on behalf of Sudipta
Bhattacharyya
Sent: Monday, August 20, 2018 6:15:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Protein is dimmer in solution, ha
Hello Zhou,
How sure are you about the space group of the solution? Any signs of
twinning or t-NCS?
Best,
Sudipta.
On Mon, Aug 20, 2018, 7:36 PM SUBSCRIBE CCP4BB Zhu Qiao <
jasonqia...@gmail.com> wrote:
> Hi All
>
> My protein is dimer both in protein buffer and crystallisation reservoir,
> whi
Monday, August 20, 2018 7:55 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Protein is dimmer in solution, hard to build two chains
in crystal structure
75% solvent is not uncommon, and such crystals often only diffract to 3A.
Eleanor
On 20 August 2018 at 10:24, SUBSCRIBE CCP4BB Zhu Qiao
mailto:ja
75% solvent is not uncommon, and such crystals often only diffract to 3A.
Eleanor
On 20 August 2018 at 10:24, SUBSCRIBE CCP4BB Zhu Qiao wrote:
> Hi All
>
> My protein is dimer both in protein buffer and crystallisation reservoir,
> which is confirmed by calibration column Supderdex 200 10/300 in
Hi All
My protein is dimer both in protein buffer and crystallisation reservoir, which
is confirmed by calibration column Supderdex 200 10/300 increase.
The crystal can diffract to 3 and the space group was determined to be P212121.
The solvent content and Matthews coefficient shows
1 copy,
