Re: [ccp4bb] Protein concentration for crystallization

2013-06-11 Thread Patrick Shaw Stewart
an spending 2 ul on prescreening. > > ** ** > > Just an opinion sans statistics, BR > > ** ** > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *Debasish > Chattopadhyay > *Sent:* Tuesday, June 11, 2013 1:02 AM > *To:* CCP4BB@JI

Re: [ccp4bb] Protein concentration for crystallization

2013-06-11 Thread Bernhard Rupp
Tuesday, June 11, 2013 1:02 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Protein concentration for crystallization Perhaps my question was not expressed well. I wanted to know if proteins crystallize more frequently when the protein concentration is in the range 5-30mg/ml. The a

Re: [ccp4bb] Protein concentration for crystallization

2013-06-10 Thread Debasish Chattopadhyay
.UK> Subject: [ccp4bb] Protein concentration for crystallization What would be a convenient way to estimate what percentages of proteins have been crystallized in a concentration range, for example 5-30 mg? Debasish Chattopadhyay University of Alabama at Birmingham CBSE-250 1025 18th Street

Re: [ccp4bb] Protein concentration for crystallization

2013-06-10 Thread rana ibd
Best Regards Rana From: Debasish Chattopadhyay To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, June 10, 2013 4:49 PM Subject: [ccp4bb] Protein concentration for crystallization What would be a convenient way to estimate what percentages of proteins have been crystallized in a concentration range, for

Re: [ccp4bb] Protein concentration for crystallization

2013-06-10 Thread Evgeny Osipov
Dear Debasish, you can use REMARK 200 field in pdb file. Sadly, this field is not mandatory so not everyone provide protein concentration info. 10.06.2013 18:49, Debasish Chattopadhyay ?: What would be a convenient way to estimate what percentages of proteins have been crystallized in a

[ccp4bb] Protein concentration for crystallization

2013-06-10 Thread Debasish Chattopadhyay
What would be a convenient way to estimate what percentages of proteins have been crystallized in a concentration range, for example 5-30 mg? Debasish Chattopadhyay University of Alabama at Birmingham CBSE-250 1025 18th Street South, Birmingham, Al-35294 USA Ph: (205)934-0124; Fax: (205)934-0480