Hi Tom,
Currently I am also working a protein which likes to be soluble aggregates. I
am surprised to known that you manage to disrupt the aggregate to dimmer. So
far I have made many truncations however without any success so far. I might
try high salts and several detergents later. Does your
Hi Tom,
Are your protein or related proteins known to dimerise?
Is the dimer perhaps the natural state, and are the high oligomers
non-specific aggregation?
That would be my first guess, knowing nothing about the protein you are
working on.
If you are absolutely certain that the protein should
Hello everyone!
I have run into a problem in a 55kD recombinant human protein crystallization
(expressed in E.Coil). The purity is pretty good. However, it behaves as high
oligomers in the buffer with 300mM NaCl and behaves as dimers with a little
high oligomers in the buffer with 2000mM (2M)
