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Dear KS,
in addition to what Mark said, you might think about why the torsion
angles are this way, i.e. there might be interactions which twist your
protein into that conformation.
Only because something is reported as off-standard does not mean it is
Also, a very small fraction of amino acids have torsion angles that are really
outside normal ranges, as demonstrated by high resolution structures.
If the density is convincing, leave them as they are.
Mark J van Raaij
Laboratorio M-4
Dpto de Estructura de Macromoleculas
Centro Nacional de Biote
Hello,
There are examples of regions (such as loops) where the electron density
is rather "poor" and does not allow to build a proper model of the
polypeptide chain (the refinement program cannot fit a satisfactory
model in that area). Hence you just know that the chain "goes through
there" a
Hi all,
I am currently validating my protein and experiencing a few issues. I am unable
to fix the torsion angles for eight residues no matter what I try. There are 3
models deposited on the PDB website belonging to the same protein. Five of the
residues’ torsion angles (of the eight) are cons
