Hi Matthias,
a few suggestions:
- use TLSMD server to define TLS groups;
- before using TLSMD do some group ADP refinement with one or two
refinable ADP per residue (you can do it in phenix.refine), so you get
less biased ADPs from previous restrained B-factor refinement;
- just out of curiosi
I dont understand it either but I have also found there are problems
with starting again from the previous end point..
If you have run TLSANL after the refinement, then the TLSOUT would be
totally inappropriate to start from..
The TLS records n the PDB file and the ANISOU records are completely
Dear BB!
I have a strange problem with TLS refinement and refmac.
In my AU there are 4 monomers of the same protein.
Based on structural subdomains I defined 3 TLS groups for each of those
monomers so that the overall TLS group number is 12.
When I do TLS and restrained refinement from the GUI u
