From: CCP4 bulletin board on behalf of Keller, Jacob
Sent: Tuesday, July 25, 2017 1:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Primer design
>No need of the whole exome. Sequencing The second PCR product will do the job
>I guess. Second PCR (from the cDNA pool
y 24, 2017 7:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Primer design
Dear Syed,
The process is very trivial to clone your gene of interest. Assuming your gene
is transcribed as mono-cistronic mRNA, take the oligodT primer as reverse
primer. First isolate the total RNA from the ti
ent: Monday, July 24, 2017 7:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Primer design
Dear Syed,
The process is very trivial to clone your gene of interest. Assuming your gene
is transcribed as mono-cistronic mRNA, take the oligodT primer as reverse
primer. First isolate the total RN
Or sequence the whole exome for what, $500-1000?
JPK
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Debasish
Kumar Ghosh
Sent: Monday, July 24, 2017 7:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Primer design
Dear Syed,
The
/computational_functional_genomics.html
- Original Message -
From: "syed ibrahim" <048c02cac012-dmarc-requ...@jiscmail.ac.uk>
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, July 24, 2017 4:56:00 PM
Subject: [ccp4bb] Primer design
Hello All
I am interested in cloning a gene from a plant
Have to do primer walking in a cdna library first.
Artem
On Jul 24, 2017 7:26 AM, "syed ibrahim" <
048c02cac012-dmarc-requ...@jiscmail.ac.uk> wrote:
Hello All
I am interested in cloning a gene from a plant. I searched the database
only partial sequence is available, ie: for 160 residues onl
Hello All
I am interested in cloning a gene from a plant. I searched the database only
partial sequence is available, ie: for 160 residues only. The full length of
the protein is around 570 residues. I designed forward primer and I have no
clue to design reverse primer.
Any help
Thank you
Sy
