Re: [ccp4bb] Poor diffraction of eukaryotic membrane protein crystals

2009-02-04 Thread Das, Debanu
Poor diffraction of eukaryotic membrane protein crystals Dear CCP4bb, I am attempting to crystallise a 25kDa membrane protein of eukaryotic origin. We have obtained crystals of the protein (with and without a potential ligand). However crystal quality is poor, as exposure at room temperature and

[ccp4bb] Poor diffraction of eukaryotic membrane protein crystals

2009-02-04 Thread Damon Colbert
Dear CCP4bb, I am attempting to crystallise a 25kDa membrane protein of eukaryotic origin. We have obtained crystals of the protein (with and without a potential ligand). However crystal quality is poor, as exposure at room temperature and cryo-protected conditions have given diffraction as