Also, saying that you have
a. 4.0 data without defining the beam line
b. plates without defining the dimensions
is of limited use to get feedback, in my view.
If you got 4.0 data at home, and your plates are at least a couple of micron
thick,
one of the few modern micro crystal beam lines is
You might get better help if you post links (not attachments) to diffraction
images from several angles and an image of the crystal that gave the image, and
maybe several images of typical crystals.
Also, you could post dimensions of the crystal, and if your lab is set up for
it, a picture of t
apart from optimising the crystallisation conditions, it might be
worth optimising the protein preparation or do some limited
proteolysis - or even express short N-terminal and/or C-terminal
deletions.
Mark
Quoting Patrick Shaw Stewart:
Jahan
It sounds as though the protein crystallizes
Jahan
It sounds as though the protein crystallizes well, so microseeding (done
the right way) is very likely to solve the problem. Make a strong seed
stock with as much crystalline material as possible from several wells,
including different hits if possible. Just mix them all together, but keep
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Dear Jahan,
is your diffraction useless, or do you call 4A resolution useless? 4A is
not too bad and can be a start for structure determination while you are
waiting for better crystals.
You give very little information about what you actually have d
M, Parthasarathy, Gopal
> wrote:
>
>> During optimization, have you tried Hampton's additive screen?
>>
>> Gopal
>>
>> From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jahan
>> Alikhajeh [ja...@graduate.o
en?
>
> Gopal
>
> From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jahan
> Alikhajeh [ja...@graduate.org]
> Sent: Monday, October 15, 2012 6:01 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Plate crystals
>
> Dear Friends,
&
cp4bb] Plate crystals
Dear Friends,
I am trying to crystalize a 70 kDa nasty protein but I got plate shape crystals
with high mosaicity and useless diffraction (up to 4A).
I tried to improve/optimize crystallization but either I got the same or
nothing. I tried seeding but I had so many crystals wi
Dear Friends,
I am trying to crystalize a 70 kDa nasty protein but I got plate shape
crystals with high mosaicity and useless diffraction (up to 4A).
I tried to improve/optimize crystallization but either I got the same or
nothing. I tried seeding but I had so many crystals without any improve
