, 2014 3:26 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Pilatus and Strategy wrt Radiation Damage
Hi All,
A major opportunity with Pilatus detectors is the chance to redistribute the
dose in reciprocal space i.e. measure a lot more data, with less dose / frame,
then decide in hindsight where
like during data collection.
> >> I think there was a publication from Diamond
> Synchrotron discussing
> >> strategies of data collection with Pilatus.
> >> We've done a little bit of systematic studies as well
> and while things
> >> may well be sample- and
xtremely slow which would be the case with thin frames and long
> >>> exposure times. In this case the speed may not remain as constant as we
> >>> would like during data collection.
> >>> I think there was a publication from Diamond Synchrotron discussing
> >&
facility-dependent, ~0.2 degree frames with ~0.2
>>> sec exposure time seemed to make good compromise between above-mentioned
>>> issues. Here I would like to emphasize again - there certainly will be
>>> samples which will benefit from somewhat different parameters.
>>
emphasize again - there certainly will be
>> samples which will benefit from somewhat different parameters.
>> Hope it helps,
>> Cheers,
>> N.
>>
>> Ruslan Sanishvili (Nukri)
>> Macromolecular Crystallographer
>> GM/CA@APS
>> X-ray Science Divisi
Wednesday, April 30, 2014 7:49 AM
To: CCP4BB@jiscmail.ac.uk
Subject: [ccp4bb] Pilatus and Strategy wrt Radiation Damage
Dear Pilatus/Radiation Damage Cognoscenti,
I read a few years ago, before the advent of Pilatus detectors,
that the best strategy was a sort of compromise between number
on behalf of Keller, Jacob
[kell...@janelia.hhmi.org]
Sent: Wednesday, April 30, 2014 7:49 AM
To: CCP4BB@jiscmail.ac.uk
Subject: [ccp4bb] Pilatus and Strategy wrt Radiation Damage
Dear Pilatus/Radiation Damage Cognoscenti,
I read a few years ago, before the advent of Pilatus detectors, that the
Dear Pilatus/Radiation Damage Cognoscenti,
I read a few years ago, before the advent of Pilatus detectors, that the best
strategy was a sort of compromise between number of images and detector readout
noise "overhead." I have heard that Pilatus detectors, however, have
essentially no readout no