Re: [ccp4bb] Perfect crystals but Low resolution

2010-07-14 Thread Mary Ho
Welcome to crystallography and the PhD. Expect a lot of failure before your degree. I know its frustrating but all of the challenges you encounter will teach you and increase your skills. I had crystals my first year, moderate resolution around 3 A and it took until end of my 4th year to phase a

[ccp4bb] Perfect crystals but Low resolution

2010-07-13 Thread Xun Lu
Dear All, I took over this project last September. Since the information in the lab are not well managed, it's kind of starting over (WRONG plasmids sequences; even WRONG oligo length/sequence in the complex which has been successfully crystallized. Those crystals only gave low resolution da