Re: [ccp4bb] PEG oxidation

2008-04-18 Thread Navdeep Sidhu
Hi Neeraj: Terese Bergfors advises several things re PEG: buy gas chromatography quality, don't mix brands/batches, store it in dark, +/- flush with nitrogen, store aliquots at -20 deg C. Reference: Terese Bergfors (ed), Protein Crystallization: Techniques, Strategies, and Tips. A Laboratory Manu

Re: [ccp4bb] PEG oxidation

2008-04-18 Thread Miller, Mitchell D.
Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Neeraj Sent: Friday, April 18, 2008 1:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] PEG oxidation Hi alli have been trying to crystallize a protein and have had some hits with conditions having PEG in them (400

[ccp4bb] PEG oxidation

2008-04-18 Thread Neeraj
Hi alli have been trying to crystallize a protein and have had some hits with conditions having PEG in them (400 and 4000).but everytime i have tried to reproduce the conditions there has been a lot of heterogeneity..and then to look for answers i delved a little deeper into underst