Dear Klaus
If your compound can be dried, you can add it to the drop as a powder
from the tip of a acupuncture needle. At least this works for many of
the insoluble heavy metal derivatives. We have also successfully
resuspended the powder in the optimal buffer of choice and added it as
insolub
Dear Klaus,
We've had a lot of luck with ethylene glycol and isopropanol, using them
successfully in cases where DMSO affects diffraction.
Best wishes
Richard
=
Dr Richard Bayliss, Reader in Structural Biology
Department of Biochemistry
Henry Wellcome Building
Univer
Hi Klaus,
- small molecular weight PEG's e.g. 200 instead of DMSO, has the advantage of
also helping to cryo protect
- Methanol (only for dispensing the compound into wells) then allow to
evaporate and simply add your cryo-protected crystals, the hope is that
sufficient of your ligand goes into
Dear CCP4BB followers,
We are currently trying to obtain ligand-bound complexes for one of our
proteins by soaking and/or co-crystallisation. We have had prior success for
this protein, but using a different class of ligands. The new ligand (in DMSO)
remains in solution (more or less) when mi
