another option might be the age of the screen perhaps ?
very silly suggestion/question: your pH meter is calibrated before you make
your measurements right ?
If you have a nano-pH probe (anything that can be added to a 96well reservoir
well) you could test what the final pH of your composition i
Hello, Nasir,
What concentration of your ammonium sulphate stock solution? Do your
solution remains immiscible without ammonium sulphate?May be addition of
concentrated solution of ammonium sulphate to PEG MME could cause phase
separation? Anyway, try to heat your solution a bit as recommended
Dear Nazia Nasir,
did you warm up the solution a little? This makes working with PEG
solutions a lot easier. You may also note the Hampton do not maintain
the pH: they use the buffers as indicated but this does not mean that
the final solution still has pH 6.5. With your ingredients it probably
is
I have obtained crystals in Hampton Crustal Screen 2, condition 26.The
condition has the following components:
1. 0.2 M ammonium sulphate
2. 0.1 M MES buffer pH 6.5
3. 30% PEG mononmethyl ether 5,000.
When I try to prepare the screen in-house, I use the Hampton stocks of 3.5
M ammonium sulphate an
