Re: [ccp4bb] Off topic: Oligonucleotide purity (Summary)

2013-03-21 Thread Theresa Hsu
Dear all I received off-board replies on this subject. Most people use standard desalting for all cloning and mutagenesis experiments regardless of length. One reply recommended desalting for up to 40 bases and HPLC for longer oligos because of larger ratio of (n-1) contaminants. For crystalliz

[ccp4bb] Off topic: Oligonucleotide purity

2013-03-17 Thread Theresa Hsu
Dear all What is the right (cost wise) purity level for DNA oligonucleotide synthesized for cloning, site-directed mutagenesis and protein/DNA co-crystallisation? Thank you. Theresa