Hi Ming,
You can find a protocol for low pH native gel electrophoresis (as well
for high pH and blue native) in the following book:
Gel Electrophoresis of Proteins: A Practical Approach (Practical
Approach Series) by B. D. Hames.
http://www.amazon.com/Gel-Electrophoresis-Proteins-Practical-A
Ming,
On that note, here is a Blue Native PAGE protocol that reportedly
improves the separation of basic proteins. A friend and I have used it
for native protein separation, but not necessarily for basic proteins.
Word of warning: don't use histidine salts or Tris-HCl in the cathode
buffer.
You could try Coomassie Blue Native gel. It's a very neat technique and it
worked for me on a couple of occasions. In one unfortunate case, it
resulted in dissociation of a heterotetrameric complex, though.
Artem
> Dear CCP4 community,
>
> Sorry for the off-topic subject, but I would really appre
Dear CCP4 community,
Sorry for the off-topic subject, but I would really appreciate some
suggestions and/or protocols relating to native gel electrophoresis of
basic proteins. I have used a general acidic PAGE protocol for my protein,
which has a PI of 9.5. Briefly, the protein was loaded onto a n
