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annie.m.hass...@gsk.com
"Darren Hart"
Sent by: "CCP4 bulletin board"
24-Mar-2009 11:22
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[ccp4bb] OT: Crystallisation compatible detergents
Hello,
We can make a nice 1:1 complex
Hi Darren
I believe that the most frequently used detergent for protein
crystallization (not including membrane proteins) is octyl-glucoside.
The most important parameter is the CMC of the detergent and the size of the
micelles of free detergent if you have micelles around. These considerations
do
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] OT: Crystallisation compatible detergents
Hi Darren,
I'm not aware of any (membrane) protein crystal structures solved with tween20.
It's heterogeneous, and its color suggests it contains impurities and/or
oxidation products, making it
/24/2009 11:22 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] OT: Crystallisation compatible detergents
Hello,
We can make a nice 1:1 complex between two proteins that then gradually
precipitates during storage at 4 degC. With 0.005% Tween-20 the complex is
stable and does not preciptate noticeably.
Hello,
We can make a nice 1:1 complex between two proteins that then gradually
precipitates during storage at 4 degC. With 0.005% Tween-20 the complex is
stable and does not preciptate noticeably. We have used this in buffers to
measure the kinetics of binding by Biacore so it is clearly compatible
