Re: [ccp4bb] OFF TOPIC: Cooling system for columns

2015-02-03 Thread Roger Dodd
Hi Chiara, We also have an Avant at room temperature. My recommendation would be to forgo using a water bath. Instead you can achieve a prep near enough to 4C by doing 2 things: Firstly, keep your columns stored in the cold room or a fridge to pre-chill them before use. Secondly, make your buffers

[ccp4bb] OFF TOPIC: Cooling system for columns

2015-02-03 Thread Chiara Rapisarda
Hello, we have an AKTA Avant that we keep at room temperature. We would like to be able to use it for preps at 4 degrees, so we would like to keep the FPLC columns (type 10/30 from GE) in the cold. We were thinking of buying a circulating water bath and connect it somehow to the columns through